Q-Bioanalytic is very active in the research & development area. We as a company believe it's important to invest
in new projects to keep the world safer. At this moment we run multiple projects with international partners spread out over the whole world. To have a small idea where we are currently working
on or worked on you can explore this page.
DIAGMAL is a collaborative research project which aims to develop a novel molecular diagnostic test for malaria to a position where market launch is possible. The project is funded by the European Commission Seventh Framework Programme for a period of three years, beginning in September 2013.
Many different forms of diagnostic test for the malaria parasite have been developed and put into use around the world with major benefits for the healthcare of millions of people. For new diagnostic tests to be successful they need to show advantages in one or more of the following areas: performance, ease of use and cost. Against this backdrop, the DIAGMAL project team intends to take to the point of market readiness a highly sensitive, easy to use and relatively low cost test. Early development and successful initial field evaluation of the test has been carried out under a now-complete EU project called MALACTRES.
The project consortium comprises organisations from The Netherlands, Germany, Finland and the UK.
As the project develops, information on activities and results will be added to the site, along with news and publications.
The project consortium welcomes comments and questions about the project.
Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA) are major zoonotic pathogens. Recent studies have demonstrated that MRSA of defined clonal lineages (predominantly the clonal complex (CC) 398) was found to colonize livestock including pigs, cattle and poultry and to reach colonization rates of 43-70% among these animals in Germany. Therefore, the term “livestock-associated MRSA” (LA-MRSA) had been introduced. The MedVet-Staph consortium was founded in 2010 to study the zoonotic impact of S. aureus/MRSA.
In its first research period (2010-2013) the MedVet-Staph consortium has demonstrated that LA-MRSA causes a significant burden of human colonization and (healthcare-associated) infection in the German population and in animals. During this period the research consortium gave 147 scientific presentations on national and international conferences and published >30 scientific articles in international peer-reviewed journals (see publications). Since 2014, the consortium continues its work in a second research period.
Entwicklung von Schnellnachweisen von Bakterien in Ballastwasser (Clean Ballast Water [CleBaWa])
Im Projekt CleBaWa werden Schnellnachweise zur Detektion von Bakterien in Ballastwasserproben von Schiffen entwickelt: sowohl ein elektrochemischer Biosensor als auch eine teststreifenbasierte Lösung. Beide Ansätze weisen die Bakterien spezifisch anhand deren DNA nach. Die Ergebnisse sollen über SmartPhone Apps den zuständigen Stellen übermittelt werden.
Dazu müssen zunächst die Bakterien aus der Ballastwasserprobe angereichert und deren DNA freigesetzt werden. Die DNA wird mittels Multiplex PCR amplifiziert und dann mittels NALFIA oder elektrochemischem Sensor nachgewiesen. Der Fokus von Hahn-Schickard liegt auf der Entwicklung dieses Sensors. Diese beinhaltet Layout, Herstellung und Aufbau des Chips, die Aufbringung der Elektroden, die Immobilisierung spezifischer DNA-Sonden und letztlich die Analyse der Proben. Die interdisziplinäre Aufgabenstellung wird in enger Zusammenarbeit der Hahn-Schickard-Standorte Freiburg (Oberflächenchemie, Elektronik, Chipherstellung) und Stuttgart (Aufbringen der Elektroden z.B. durch Inkjet Druck) durchgeführt.
|Laufzeit:||01.03.2015 bis 28.02.2018|
Hochschule Bremerhaven, Q-Bioanalytic GmbH, Barum Internetservice GbR, Alfred-Wegener-Institut, APM-Gehäusetechnik GmbH
Soon we provide more info about this project.
Although present in the water column, Vibrio (V.) spp. appear at particularly high densities in association with marine bivalves, which implicates bivalves as important zoonotic agents of pathogenic Vibrio spp. Among them are serious human pathogens, such as V. parahaemolyticus and V. vulnificus. The recent emergence of new highly pathogenic V. parahaemolyticus clones and their pandemic spread raise the question for the environmental and genetic determinants leading to the emergence, proliferation, and transmission of Vibrio spp. pathotypes and for the potentially underestimated risk to the human population in Europe and worldwide. Vibrioses are commonly reported in the USA and in many Asian and South American countries; however, there is growing concern that Vibrio spp., particularly V. parahaemolyticus and V. vulnificus, may represent an important and increasing clinical problem in Europe. Since 1996, the worldwide incidence of V. parahaemolyticus infections has increased dramatically causing large-scale outbreaks in North America, Chile, India, Southeast Asia, Japan, and more recently in Spain, France and Italy.
Moreover the aquacultre producing large quantities of shrimps, mussels and other seafood is affected by Vibrio species. In terms of aquatic animals Aliivibrio salmonicida, V. tubiashii, V. anguillarum, V. splendidus and V. vulnificus have importance.
Project title: DEVELOPMENT AND VALIDATION OF DNA BASED PROTOTYPES FOR DETECTION OF ALLERGENIC SPECIES
Summary of the project:
The project consist of the following phases (WP):
i) project management and partner activities organisation and monitoring;
ii) Implementation of Allergenic species Detection Prototypes (ADPs). Three different prototypes will be considered for implementation: 1) Multiplexed PCR ADP prototype (MX-PCR-ADP), a Real time PCR multiplexed for the simultaneous detection of different allergenic species (up to 6) and with the SybrGreen chemistry 2) Colorimetric Microarray prototype (C-M-ADP), a low density microarray with a colorimetric detection system, and 3) a ready-to-use 96 microtiter plate containing single-plex PCR primers/probes prototype (MT-S-PCR-ADP).
iii) preparation of reference materials (DNA) validated in ring tests between the partners of the consortium. The reference materials will be prepared for different plant and animal species according to labelling regulations in Europe. Condition of their storage and shipping will be tested.
iv) challenging activities on matrices from real samples containing allergenic species. The aim is to test the “fitness-for-purpose” of the developed ADPs. Results will show the applicability of the developed ADPs and their possibilities and limits.
v) development of a business plan and market analysis. The functional and the technical properties of the product will be defined and expressed. A Market study will be conducted mainly in Europe with little investigation in USA and Asia. The Exploitation plan will comprise the definition of a global turnover of the product, the definition of the position of the product versus competition, and the business model (nature of the revenues and of the costs).
The innovation potential is an extensive breakthrough in traceability through the application of DNA technology: array and PCR for allergenic species detection. The results achieved will bring the use of these technologies and of the relative chemistries well beyond the actual level.
Portable microfluidic-based device for in situ detection of viable Legionella.
Legionella outbreaks have great sanitary, economic and social implications. Diagnostic method use to prevent and control Legionella is bacteria culture. Available detection kits are based on DNA technology, unable to discriminate between viable and non-viable bacteria. On the contrary, RNA is widely used as a viable bacteria indicator. Although molecular based techniques, as PCR and qPCR, have become essential tools in genetic analyses due to its high sensitivity and accuracy, they show drawbacks as time-consuming and expensive. Recent development of integrated microfluidic systems has allowed time, volume and analysis cost reduction. An important application of microfluididc systems is the ability to efficiently process raw samples and perform the required analytical assays “on-chip”. There is no portable system to detect viable Legionella on-site in the market. This device will avoid sample pre-treatment therefore reducing data acquisition. Moreover, rapid result gathering will allow implementing a rapid plan of action for bacteria eradication. The project aims to develop a portable microfluidic device composed of a filtration and bacteria collection system and a disposable card (containing a microfluidic chamber for nucleic acids concentration, a microfluidic chamber for RNA amplification through NASBA, and a detection system based on a NALFIA strip). An essential part of PINVIALEG is the development of a portable device where the filtration system is integrated to a microfluidic based cartridge. Thus, the whole process of Legionella detection (from sample collection to results) can be performed on site. NASBA is an isothermal process, facilitating portable equipment production. Development of a NALFIA strip able to detect NASBA products as a reading system will make the device very simple to use by non-qualified personnel. A new innovative tool will be produced to detect viable Legionella on-site, with rapid result acquisition and easy to use.
Funded by the European Commission in the 6th Framework Programme for Research (FP 6) the CHILL-ON project aims to improve quality, safety, transparency and traceability of the chilled/frozen supply chain. To reach the goal, cost-effective technologies, devices and approaches for continuous monitoring and recording of relevant data and processing the data for information management throughout the entire supply chain are under way. As fish and poultry are very prominent in the European food market and belong to the most sensitive goods with regard to food poisoning, the chilled and frozen fish and poultry supply chain has been selected as test cases.
CHILL-ON aims to improve the quality, safety and transparency of the food supply chain. Through a proactive and integrated approach, CHILL-ON will implement in-depth research for each step of the chilled/frozen supply chain.
The outputs of this project are integrated in a holistic approach to deliver a complete solution, to significantly enhance the state of the art of the entire food supply chain from - "farm to fork". The project will result in a novel concept for tracking and tracing - "TRACECHILL" to include input from novel biosensors for low temperature micro organisms, novel chilling and packaging technologies and smart labelling.
The focus of the CHILL-ON project is to provide for the first time ever an integrated solution for the entire frozen and chilled food supply chain. This will be done by implementing an interdisciplinary research and development work programme which includes a very wide spectrum of disciplines: from biochemistry; genetics and microbiology, through chilling, packaging, smart labels (eChillOn) and logistics up to information technologies such as active and passive RFID, GPS, GIS, electronic Supply Chain Management (eSCM) and a Decision Support System (DSS). This is integrated into the novel traceability solution proposed by CHILL-ON for the frozen/chilled food supply chain called TRACECHILL.